The story of Biwi Ho ToAisi revolves around the tarmented relationship between a mother in law and her newly arrived daughter in law. Mrs. KamlaBhandari, a rich business woman of the modern world has her own design to set up her house on western line, whereas the poor daughter in law Shaloo, an un educated village girl believes in total Indian culture and traditions. More over, with the arrival of Shaloo in the house KamlaBhandari's cherished dream to multiply her wealth with fabolous amounts of expected dowries by getting her two sons married elsewhere is crushed to ashes, so she very strongly condemnsShaloo from the very beginning and refuses to except her in the house but Shaloo does live in the same house and performs her duties to the best of a true Indian daughter in law. Irritated with this, Mrs. KamlaBhandari with the help of her opportunist secretary lands to get rid of Shaloo several times one after the other. But everytime their plan flops on her and Shaloo goes on winning the hearts of the other members of Bhandari family till a big theft charge is successfully planted on her and she is turn out of the house but soon her innocence proved and her husband SurajBhandari restores her back in the house.
The events following her restoration in the house, are the part of a big CLIMAX, which blasts a bomb shell of surprise for everybody and brings all the differences settled between the mother in law and the daughter in law on a very very happy note.